https://friveroll.github.io/tags/fluorescencia/ Recent content in Fluorescencia on Felipe Riveroll Aguirre Hugo -- gohugo.io es Sat, 04 Apr 2026 00:00:00 +0000 https://friveroll.github.io/posts/western-blotting-parte-iv-deteccion-imagen/ Sat, 04 Apr 2026 00:00:00 +0000 https://friveroll.github.io/posts/western-blotting-parte-iv-deteccion-imagen/ <p>Tras completar la inmunodetección con anticuerpos primario y secundario, el siguiente paso crítico en el western blot es la detección e imagen de la señal. Esta etapa transforma la unión anticuerpo–antígeno en una señal cuantificable, y la elección del método de detección, del sustrato, del sistema de revelado y de los parámetros de adquisición condiciona directamente la posibilidad de interpretar cuantitativamente los datos <span class="hugo-cite-intext" itemprop="citation">(<span class="hugo-cite-group"> <a href="#thermo2024quantitative"><span class="visually-hidden">Citation: </span><span itemprop="author" itemscope itemtype="https://schema.org/Person"><span itemprop="familyName">Thermo Fisher Scientific</span></span>,&#32;<span itemprop="datePublished">2024</span></a><span class="hugo-cite-citation"> <span itemscope itemtype="https://schema.org/WebPage" data-type="webpage"><span itemprop="author" itemscope itemtype="https://schema.org/Person"><span itemprop="familyName">Thermo Fisher Scientific</span></span>(<span itemprop="datePublished">2024</span>).&#32;Retrieved from&#32; <a href="https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/quantitative-western-blot-analysis.html" itemprop="identifier" itemtype="https://schema.org/URL">https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/quantitative-western-blot-analysis.html</a></span> </span></span>;&#32;<span class="hugo-cite-group"> <a href="#taylor2013simple"><span class="visually-hidden">Citation: </span><span itemprop="author" itemscope itemtype="https://schema.org/Person"><meta itemprop="givenName" content="S. C."><span itemprop="familyName">Taylor</span></span>&#32;&amp;&#32;<span itemprop="author" itemscope itemtype="https://schema.org/Person"><meta itemprop="givenName" content="A."><span itemprop="familyName">Posch</span></span>,&#32;<span itemprop="datePublished">2013</span></a><span class="hugo-cite-citation"> <span itemscope itemtype="https://schema.org/Article" data-type="article"><span itemprop="author" itemscope itemtype="https://schema.org/Person"><span itemprop="familyName">Taylor</span>,&#32; <meta itemprop="givenName" content="S. C." /> S.</span>&#32;&amp;&#32;<span itemprop="author" itemscope itemtype="https://schema.org/Person"><span itemprop="familyName">Posch</span>,&#32; <meta itemprop="givenName" content="A." /> A.</span> &#32; (<span itemprop="datePublished">2013</span>). &#32;<span itemprop="name">The design of a quantitative western blot experiment</span>.<i> <span itemprop="about">Methods</span>,&#32;61(1)</i>.&#32;<span itemprop="pagination">11–19</span>. <a href="https://doi.org/10.1016/j.ymeth.2013.06.015" itemprop="identifier" itemtype="https://schema.org/URL">https://doi.org/10.1016/j.ymeth.2013.06.015</a></span> </span></span>)</span> .</p>